SPR Technology (关于表面等离子共振技术的原理结构及其应用)
Surface Plasmon Resonance (SPR) has emerged as a powerful technique in life science research, drug discovery, electroanalysis, food quality and safety, environmental science and chemical sensor development. The key of the technique is to excite and detect collective oscillations of free electrons in a metal film referred to as surface plasmons. The excitation and detection can be achieved using several different configurations. A widely used one is the Kretschmann configuration, in which light is focused onto the metal film through a glass prism and the reflection of the light is detected (Fig. A). At a certain incident angle (resonance angle), the plasmons are set to resonate with light, resulting in absorption of light at that angle and thus a dark line in the reflected beam (Fig. B). A molecular binding event taking place on or near the metal film, or a conformational change in the molecules bound to the film causes a shift in the resonance angle. By monitoring the shift vs. time, molecular binding events can be monitored and kinetics of the binding events can be studied without labels (Fig. C). 表面等离子体激元共振 (SPR) 已成为生命科学，药物开发，电分析，食品质量和安全检测，环境科学以及化学传感器等领域中一种强有力的分析技术。该技术的核心是通过激发和检测金属膜中自 由电子的振动，即所谓的表面等离子体来进行检测。一种广泛使用的构型为Kretschmann构型, 激光通过玻璃棱镜聚焦到金属膜上，然后检测反射的激光（图A）。 在一定的入射角（共振角），等离子体发生共振，光在该角度产生吸收，从而导致反射光束黑线的产生（图B）。金属膜上发生的分子键合或膜上的分子构型变化导致共振角发生位移。通过检测位移与时间的关系曲线，可以免标记检测分子间的键合反应以及动力学参数（图C）。
BI-SPR Technology
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Injection SPR . Electrochemical
SPR
Gas Phase SPR .
Technical Notes
